This study investigates the efficient de-fluoridation of wastewater using activated biochar and provides a comprehensive thermodynamic and kinetic assessment. Fluoride contamination in water sources is a pervasive issue with adverse health implications. Activated biochar, derived from sustainable biomass sources, has shown promise as an eco-friendly and cost-effective adsorbent for fluoride removal. Batch experiments were conducted to evaluate the influence of temperature, initial fluoride concentration, contact time, and biochar dosage on the de-fluoridation process. Thermodynamic parameters, including Gibbs free energy (∆G°), enthalpy (∆H°), and entropy (∆S°), were determined to elucidate the spontaneity and nature of the de-fluoridation process. The kinetic data were analyzed using various models to unveil the underlying mechanisms. This study provides valuable insights into the feasibility and effectiveness of activated biochar for wastewater de-fluoridation, with implications for water treatment and environmental remediation.