This study offers insights into the surface chemical changes that occur during the silanisation process of borosilicate glass covers. Silanisation, a crucial step in functionalizing glass surfaces for various applications, involves the covalent attachment of organosilane molecules. Through a comprehensive monitoring study, the chemical transformations on the glass surface during silanisation were investigated using various analytical techniques. The results shed light on the formation of silane monolayers, changes in surface wettability, and the impact of reaction parameters on surface modification. These findings contribute to a deeper understanding of the silanisation process and its potential applications in biotechnology, materials science, and microfabrication.