This article presents the development of a method for determining mephedrone and methamphetamine using high-performance liquid chromatography (HPLC). Research was conducted to develop an HPLC method for analyzing mephedrone and methamphetamine, which are included in the list of prohibited narcotic substances in the Republic of Uzbekistan. A method for extracting and identifying mephedrone and methamphetamine from physical evidence was developed. The linearity of the method was determined.

Under the selected analytical conditions of the HPLC method, the retention time of mephedrone was determined to be 5.158 minutes, while the retention time of methamphetamine was 4.320 minutes. This analytical method was confirmed to be specific for mephedrone and methamphetamine.

amphetamines, mephedrone, methamphetamine, high-performance liquid chromatography, linearity, standard sample, retention time, chromatographic peak.

Fitsev, I.M., Vlasova, O.V., Rizvanov, I.Kh., Fitseva, N.A., Budnikov, G.K. Gladyrev, V.V.Stepushchenko Analytical Profiles of Beta-Ketoamphetamines. II. Identification Using Thin-Layer Chromatography, Gas Chromatography, IR, and UV Spectroscopy. 2010, pp. 11-12.

Ramenskaya, G.V., Rodionova, G.M., Kuznetsova, N.I., Petukhov, A.E.

TLC-Screening of Toxicologically Significant Compounds Isolated by Extraction and Sorption: A Study Guide. 2018, pp. 14-18.

J.Musaev, UamonovA.B. Combating Drug-Related Crimes in Cyberspace. Scientific Progress, Vol. 3, Issue 2, 2022. ISSN: 2181-1601, pp. 1161-1175.

United Nations Office on Drugs Crime (UNODC), The challenge of new psychoactive substances. 2013, Global Synthetics Monitoring: Analyses, Reporting and Trends (SMART) Programme

Schifano, F., A. Albanese, S. Fergus, J.L. Stair, P. Deluca, O. Corazza, Z. Davey, J. Corkery, H. Siemann, N. Scherbaum, M. Farré, M. Torrens, Z. Demetrovics, A.H. Ghodse, P.W. Mapping, and R.R. Groups, Mephedrone (4-methylmethcathinone; “meow meow”): chemical, pharmacological and clinical issues. Psychopharmacology (Berl), 2011. 214(3): p. 593-602

UNODC. World Drug Report 2023. United Nations Office on Drugs and Crime, Vienna, 2023.