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https://doi.org/10.55640/
COMPREHENSIVE HPLC ANALYSIS OF WATER-SOLUBLE B-GROUP AND VITAMIN C CONTENTS IN LIMONIUM GMELINII (ARSLONQUYRUQ)
Obidov Ulugbek Mirpozil ugli¹, Jalilova Sevarakhon Tavakkaljon kizi² , 1.Undergraduate Student, Andijan Branch, Kokand University, Uzbekistan Lecturer, Andijan Branch, Kokand University; PhD Candidate (Basic Doctoral Student), Andijan State University, UzbekistanAbstract
In this study, the content of water-soluble B-group vitamins (B₁, B₂, B₆, B₉, B₁₂) and ascorbic acid (vitamin C) in the aqueous extract of Arslonquyruq (Limonium gmelinii) was determined using high-performance liquid chromatography (HPLC), and their practical significance in the fields of medicine and pharmacology was comprehensively evaluated. It is well known that water-soluble vitamins play a crucial role in essential metabolic and physiological processes in the human body, including regulation of nervous system function, strengthening of the immune system, hematopoiesis, and enhancement of antioxidant defense mechanisms. Therefore, the investigation of the vitamin composition of medicinal plant extracts using modern analytical techniques is of particular relevance and importance in contemporary scientific research. During the study, the precise concentrations of vitamins in the extract were determined using a Shimadzu LC-40 Nexera Lite HPLC system in combination with internationally recognized standard vitamin solutions. The results revealed that the Arslonquyruq extract contained high levels of vitamin C and B-group vitamins, substantiating the potential of this plant for extensive application in the pharmaceutical and dietary product industries as a natural source of antioxidants and bioactive compounds. In particular, the vitamin C content was recognized for its essential role in collagen synthesis, suppression of inflammatory processes, and activation of the immune system, while B-group vitamins were found to play a critical role in nervous system function and hematopoiesis. Overall, the findings indicate that Limonium gmelinii is a promising bioactive medicinal plant with significant potential for use in healthcare, pharmaceutical sciences, nutrition, and phytotherapy. In addition, the feasibility of developing natural antioxidant preparations with high biological activity, as well as vitamin-enriched beverages and nutraceutical products based on Arslonquyruq extract, was scientifically substantiated. This study contributes to an in-depth investigation of medicinal plants native to the republic and expands the possibilities for their utilization in the domestic pharmaceutical and food industries.
Keywords
Arslonquyruq (Limonium gmelinii), water-soluble vitamins, vitamin C, B-group vitamins, high-performance liquid chromatography (HPLC), biological activity, antioxidant, pharmacology, plant extract, Shimadzu LC-40 Nexera Lite.
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