The present study investigates the influence of cobalt(II) and manganese(II) nitrate-based hydrogels on the antioxidant activity of Matricaria chamomilla L. (German chamomile), a medicinal plant widely recognized for its pharmacological and therapeutic importance. Hydrogels were synthesized using a polyacrylamide-based matrix incorporating controlled concentrations of Co²⁺ and Mn²⁺ ions to facilitate the gradual release of micronutrients into the rhizosphere. The experimental design included control plants and treatment groups exposed to hydrogels enriched with cobalt(II) nitrate and manganese(II) nitrate, respectively. The antioxidant potential of M. chamomilla extracts was assessed using the DPPH and FRAP assays, while total phenolic and flavonoid contents were determined spectrophotometrically. Results indicated that hydrogel-mediated delivery of cobalt and manganese ions significantly enhanced the antioxidant capacity of chamomile tissues compared with untreated controls. The increase in antioxidant activity was accompanied by elevated levels of phenolic compounds, suggesting the activation of enzymatic and non-enzymatic defense pathways in response to the micronutrient supplementation. These findings highlight the potential application of transition-metal-based hydrogels as slow-release micronutrient carriers to improve the biochemical quality and medicinal value of aromatic and pharmaceutical plants under controlled cultivation conditions.

Matricaria chamomilla L.; cobalt(II) nitrate; manganese(II) nitrate; hydrogel; antioxidant activity; DPPH assay; FRAP assay; phenolic compounds; medicinal plants; micronutrient delivery.

Saitkulov, F., Begimqulov, I., Oʻralova, N., Gulimmatova, R., & Rahmonqulova, D. (2022). Biochemical effects of the coordination compound of cobalt-II nitrate quinazolin-4-one with 3-indolyl acetic acid in the “amber” plants grades phaseolus aureus. Академические исследования в современной науке, 1(17), 263-267.

Saitkulov, F., Qilichyeva, N., Abdullayev, B., Anvarov, A., & Ergasheva, M. (2022). Titrimetric analysis of calcium cation in" megaton" variety of cabbage. International Bulletin of Applied Science and Technology, 2(10), 134-135.

Хайдаров, Г. Ш., Тилябов, М. У., Холмирзаев, М. М., & Элмурадов, Б. Ж. Синтез и биологическая активность гидрохлорид хиназолин-4-она. Fan va taʼlim integratsiyasi” jurnalining Tahrir hay’ati tarkibi.

Saitkulov, F., Farhodov, O., Olisheva, M., Saparboyeva, S., & Azimova, U. (2022). Chemical feeding method of lemon plant using leaf stomata. Академические исследования в современной науке, 1(17), 274-277.

Саиткулов, Ф. Э., & Элмурадов, Б. Ж. (2022). УФ-спектральные характеристики хиназолин-4-он и–тионов. In Innovative developments and research in education international scientific-online conference. pp-10-12.

Azamatova, M., Meliyeva, S., Azamova, S., Sapaev, B., & Saitkulov, F. (2023). Healing properties of chamomile. Академические исследования в современной науке, 2(8), 37-40.

Sapaev, B., Saitkulov, F., Mamedova, M., Saydaliyeva, S., & Makhmudova, D. (2023). Chromato-mass-spectrometry of the analysis of the sum of the common mushrooms. In BIO Web of Conferences (Vol. 65, p. 01006). EDP Sciences.

Amirova, N., Qulmaxamatova, D., Bebitova, K., Saitkulov, F., & Nasimov, K. (2023). Technology of creating cool beverages rich in vitamins based on rose hip fruit. Theoretical aspects in the formation of pedagogical sciences, 2(5), 169-172.

Saitkulov, F. E., & Elmuradov, B. J. (2022). UV spectral characteristics of quinazoline-4-one and thions. In Innovative developments and research in education international scientific-online conference (pp. 10-12).

Saitkulov, F., Xudayarov, M., Eshboboyev, T., Oxunov, I., & Amanov, R. (2025, July). Coordination compounds of manganese salt with acetamide and study of biochemical properties of the cotton variety" Sultan" plant. In American Institute of Physics Conference Series (Vol. 3304, No. 1, p. 040098).

Kulmirzayeva, S., Isaqulova, M., Nasimov, H., Saitkulov, F., & Islomova, D. (2025, July). Study of synthesis and biological properties of coordination compound of cobalt (II)-chloride. In American Institute of Physics Conference Series (Vol. 3304, No. 1, p. 040099).

Zulpanov F. A., Saitkulov F. E., Elmuradov B. J., Arzanov R. X. (2024). Study of Benzylation Reactions of Quinazolin‑4‑One in the Presence of Various Solvents. Austrian Journal of Technical and Natural Sciences 2024, No 3 – 4. https://doi.org/10.29013/AJT-24-11.12-52-56

Fayzullozoda H.M., Khaydarov G.Sh., Baymuratova G.O., Saitkulov F.E. (2024). Synthesis and Study of Biological Activity of Coordination Compounds Based on Copper (Ii) Nitrate and Quinazolin-4-One. Austrian Journal of Technical and Natural Sciences 2024, No 3–4. https://doi.org/10.29013/AJT-24-11.12-43-46

Ashurova Z. B., Khaydarov G. Sh., Saitkulov F. E., Giyasov K. (2024). Determination of Certain Heavy Metals in Food Composition by Voltammetric Method. Austrian Journal of Technical and Natural Sciences 2024, No 3 – 4. https://doi.org/10.29013/AJT-24-11.12-47-51

Saitkulov F., Qayumova F. Synthesis of coordination compounds based on cobalt(ii) salts and quinazolin-4-one and the study of their biological activity // Universum: химия и биология : электрон. научн. журн. 2025. 2(128). URL:https://7universum.com/ru/nature/archive/item/19240

Foziljon, S., & Fotima, Q. (2025). Synthesis of coordination compounds based on cobalt (ii) salts and quinazolin-4-one and the study of their biological activity. Universum: химия и биология, 2(2 (128)), 38-42.

Akhmedova, N., Elmuradov, B., & Saitkulov, F. (2024). Optimal synthesis methods and biological activity study of 2-(4-nitrophenil) quinazolin-4-one. Multidisciplinary Journal of Science and Technology, 4(12), 1192-1200.

Shoyimovich, K. G., Ergashevich, S. F., & Kuchkar, G. (2024). DETERMINATION OF CERTAIN HEAVY METALS IN FOOD COMPOSITION BY VOLTAMMETRIC METHOD. Austrian Journal of Technical & Natural Sciences.