This article explores the hormonal changes that occur in the human body during stress and the role of laboratory diagnostics in identifying these alterations. The study focuses on the activity of the hypothalamic-pituitary-adrenal (HPA) axis and evaluates levels of cortisol, adrenocorticotropic hormone (ACTH), thyroid, and reproductive hormones. Modern laboratory methods, including enzyme-linked immunosorbent assay (ELISA) and chemiluminescent analysis, were used to assess the physiological responses to stress. The results indicate that prolonged exposure to stress leads to hormonal imbalance, suppression of immune function, and the development of metabolic disorders. The paper emphasizes the importance of early detection of stress-induced hormonal changes and highlights the diagnostic value of laboratory testing in the prevention and management of stress-related conditions.

Stress, hormones, cortisol, ACTH, hypothalamic-pituitary-adrenal axis, laboratory diagnostics, endocrine system, physiological changes, ELISA, hormonal imbalance.

Chrousos, G. P. (2009). Stress and disorders of the stress system. Nature Reviews Endocrinology, 5(7), 374–381.

McEwen, B. S. (2007). Physiology and neurobiology of stress and adaptation: Central role of the brain. Physiological Reviews, 87(3), 873–904.

Tsigos, C., & Chrousos, G. P. (2002). Hypothalamic–pituitary–adrenal axis, neuroendocrine factors, and stress. Journal of Psychosomatic Research, 53(4), 865–871.

Kudielka, B. M., & Kirschbaum, C. (2005). Sex differences in HPA axis responses to stress: A review. Biological Psychology, 69(1), 113–132.

Sapolsky, R. M., Romero, L. M., & Munck, A. U. (2000). How do glucocorticoids influence stress responses? Endocrine Reviews, 21(1), 55–89.

Hellhammer, D. H., Wüst, S., & Kudielka, B. M. (2009). Salivary cortisol as a biomarker in stress research. Psychoneuroendocrinology, 34(2), 163–171.

Miller, G. E., Chen, E., & Zhou, E. S. (2007). Chronic stress and the hypothalamic–pituitary–adrenocortical axis in humans. Psychological Bulletin, 133(1), 25–45.

Тухтаев, М. М., & Абдуллаева, Ш. С. (2021). Gormonal tizim va stress omillarining o‘zaro ta’siri. Tibbiyotda innovatsiyalar jurnali, 3(5), 45–52.

Қосимов, А. А., & Рахматуллаев, Ф. Б. (2020). Stress holatlarida endokrin o‘zgarishlarning laborator tahlili. O‘zbekiston tibbiyot jurnali, 2(1), 67–73.

Исамиддинова, Н. Н. (2022). Kortizol darajalarining stress diagnostikasidagi ahamiyati. Toshkent tibbiyot akademiyasi ilmiy axboroti, 4(3), 58–63.

Мамадалиев, Б. Х., & Турсунова, З. Р. (2021). Psixologik stress va gormonal javob mexanizmlari. Endokrinologiya va laborator tibbiyot jurnali, 5(2), 39–46.