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| Open Access |
https://doi.org/10.55640/
EVALUATION OF THE APOPTOTIC EFFECT OF THE NATURAL COMPLEX "ASZAFIR" IN SYNERGY WITH DOXORUBICIN (COMBINATIONAL MODEL)
Mamatova Iroda Yusupovna , Doctor of Medical Sciences, Head of the Department of Biological Chemistry, Andijan State Medical Institute, Andijan, Uzbekistan Mamajonov Zafar Abdujalilovich , Department of Anatomy and clinical anatomy, Andijan State Medical Institute, Andijan, Uzbekistan Ulugbekova Gulruh Juraevna , Department of Anatomy and clinical anatomy, Andijan State Medical Institute, Andijan, Uzbekistan Askarov Ibragim Rahmonovich , Doctor of Chemical Sciences, Professor at Andijan State University, Andijan, UzbekistanAbstract
This study aimed to evaluate the apoptotic effect of the natural phytocomplex "AsZafIr" in synergy with doxorubicin on seminoma cells. Under in vitro conditions, primary cells isolated from human testicular seminoma were treated with 1 µM doxorubicin and 0.005% AsZafIr, both individually and in combination. The level of apoptosis was determined using Annexin V-FITC/PI flow cytometry, and the combinational effect was evaluated via the Synergistic Index (CI) based on the Chou–Talalay model.
The results showed that in the combination of doxorubicin and AsZafIr, the total apoptosis rate was 1.8–2 times higher compared to monotherapy groups, and the calculated Combination Index (CI = 0.78 ± 0.04) confirmed the presence of a distinct synergistic effect. The minimal proportion of necrotic cells (≤4%) indicated the relative safety of this combination.
It was noted that due to the modulation of purinergic signaling pathways (CD39/CD73/A₂A) by bioactive substances (rosmarinic acid, apigenin, luteolin, and chlorogenic acid) contained in the "AsZafIr" phytocomplex, immunosuppression in the tumor microenvironment decreased, and mitochondrial apoptotic pathways were activated. Simultaneously, a reduction in chemotherapy toxicity and the maintenance of intracellular stability were observed due to antioxidant effects.
In conclusion, the natural complex "AsZafIr" demonstrated potent synergistic apoptotic activity in combination with doxorubicin and can be recommended as a promising, relatively safe, and effective adjuvant agent for integrative oncotherapy in seminoma and other tumor types.
Keywords
AsZafIr, doxorubicin, synergy, apoptosis, seminoma, phytocomplex, purinergic signal, Annexin V/PI, Chou–Talalay model.
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